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Molecular Identification of Fungi causing Superficial Mycoses
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| Author:
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SOM J.LAKHANI, SIDDHI K CHAVAN, RASHMI MAHAJAN, DEVARSHI U GAJJAR
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| Abstract:
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Superficial mycotic infections of skin are most often caused by dermatophytes and can occur due to yeasts and non-dermatophyte molds also (NDM). Identification of the specific causative organism is important because some organisms are less likely to respond to certain antifungal agents. The present study was conducted to know the various fungal species causing cutaneous mycoses in our region. Skin scrapping samples (n=87) were collected from infected area of patients with superficial mycoses and proceeded for diagnosis by KOH mounts and mycological cultures. All samples were positive for the presence of fungal hyphae when KOH mounted slide was observed under light microscope. However, only (n=67) samples showed growth of fungi on Potato Dextrose agar (PDA). All 67 samples were then identified using microbiological and molecular techniques. The microbiological techniques included plate and spore morphologies and molecular methods included amplification and sequencing of ITS region. The number of dermatophytes isolated were 11.8% (n=8); while the non -dermatophytes (82.2%; n=59) contributed to majority of the pathogens. The dermatophytes isolated were Trichophyton spp. (n=6) and Epidermophyton spp. (n=2)while; the non-dermatophytes isolated were Aspergillus spp.(n=22), Cladosporium spp. (n=8), Curvularia spp. (n=7), Alterneria spp. (n=3), Penicillium spp. (n=6), Mucor spp. (n=2), Candida spp. (n=3), Lasiodiplodia spp. (n=2), Chaetomium spp. (n=3), Glomastixspp. (n=1), Pseudopithomyces spp. (n=1) and Didymellaceae spp.(n=1). This study highlights the importance of non-dermatophytes in causing superficial mycoses and ITS based molecular identification of pathogenic fungi. Multiple site infection, previous use of topical and non-topical corticosteroids and poor hygiene were the risk factors in patients studied.
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| Keyword:
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non-dermatophytes, dermatophytes, KOH test, internal transcribed spacer (ITS) sequencing.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.465
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| Download:
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