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Effects Antihiperglycemic of Sembung (Blumea balsamifera L) Leaf Ethanol Extract on Blood Glucose and Pancreas Histopathology in Hyperglycemic Male Wistar Rat (Rattus norvegicus)
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| Author:
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SRI WAHJUNI, NI WAYAN BOGORIANI, HAFSIAH, AHMAD FUDHOLI
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| Abstract:
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Objective: This study to determine the effect of sembung leaf ethanol extract in reducing blood glucose and histopathological change in hyperglycemic Wistar rats, includes the maintenance of test animals, fractionation and purification using TLC, column chromatography, and LCMS/MS, free radical scavenging activity of 1,1-diphenyl-2-picrillhidrazil (DPPH) as well as experimental methods with pre-post test control group design.
Methods: 20 male Wistar rats (Rattus norvegicus) were divided into five groups, the negative control group (aquades), the positive control (glibenclamide 0.18 mg/ day/ 200gBB) and the test group with varying doses/ kgBW and 150mg/ kgBW for 14 days and carried out measurement of blood sugar levels in rats. Rats collected data were analyzed using the One Way ANOVA test and continued with the LSD test.
Results: the result of LCMS management is flavonoid composition consisting of syringetin with mz = 347. In the free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethylacetate fraction of Blumea balsamifera L leaf extract IC50 value is 0.5369 µg/mL or 536.9 ppm. Data on rat blood regression levels were analyzed using the One Way ANOVA test and continued with the LSD test each showing a value of p = 0.000. This value indicates a significant difference between each group (p<0.05).
Conclusion: This research concludes that the ethanol fraction of sembung leaves can reduce protein levels in male wistar hyperglycemia rats. The secondary metabolism of syringetin contained in sembung leaves which contains antioxidants.
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| Keyword:
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Blumea balsamifera L leaf, ethanol extract, antihyperglycemic test, male Wistarrats.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.399
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| Download:
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