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The Expression of Immunohistochemistry IL-10 And IL-18 On Helminth-Infected Mice Treated With The Extract Of Areca catechu L.
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| Author:
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ENDY JULI ANTO, AZNAN LELO, SYAFRUDDIN ILYAS, MARLINE NAINGGOLAN
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| Abstract:
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Research conducted on T. muris worm infections has shown us how the immune system induces parasitic expulsion so that it can be applied more broadly to the development of the latest therapeutic STH infections. Research and development of natural and traditional ingredients for alternative anthelmintic drugs has been carried out as an anti-inflammatory and to prevent chronic infections in the colon. One of the alternative medicinal plants chosen is Areca catechu L. (A. catechu L.) seeds. This study aims to determine the effectiveness of ethanol extract and ethyl acetate fraction of areca nut on immunohistochemistry (IHC) IL-10 and IL-18 levels in the colon of male mice orally treated with T. muris infective eggs.This study is an experimental study with a post-test only control group design in male mice (Mus musculus) given 200 T. muris infective eggs. The treatment groups are the administration of 100 mg/ kg BW and 150 mg/ kg BW of ethanol extract of areca nut, as well as 100 mg/kg BW and 150 mg/kg BW ethyl acetate fraction. Each treatment was tested for IL-18 and IL-10 IHC levels.The IHC concentrations of IL-9 and IL-18 of colon tissue showed significant differences in 7 groups (p<0.05). Meanwhile, IL-10 IHC concentration showed no significant difference. Ethanol extract of areca nut with a dose of 150 mg/kg b.w. showed a more effective result than the ethyl acetate fraction of 150 mg/kg b.w. and 100 mg/kg b.w.Apart from being anthelmintic for T. muris infections, it is concluded that the ethanol extract of areca nut can be used as an anti-inflammation. This makes the use of the ethanol extract of areca nut as one of the alternative treatment options.
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| Keyword:
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Anthelmintic, Areca catechu, interleukin, Trichuris muris, worm parasites
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.370
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| Download:
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