|
Evaluation of the Cytocompatibility of Novel Gingival Retraction Pastes based on Boswellia papyrifera
|
|
| Author:
|
HIBA A. SALMAN, MANHAL A. MAJEED, EAMAN A. AL-RUBAEE
|
| Abstract:
|
This in vitro study aimed to evaluate the cytocompatibility of two novel gingival retraction pastes based on a herbal astringent agent, Boswellia papyrifera (Bp), with and without aluminium chloride (AlCl3), (5% Bp +7.5% AlCl3), and (5% Bp) respectively, and to compare them with conventional gingival retraction paste based on 15% aluminium chloride (Astringent gingival pastes, 3M ESPE) as control at a different time of exposure (2 min, 10 min, and 24 hrs) using Methyl Thiazolyl Tetrazolium (MTT assay). The cell line used was Human Dermal Fibroblast, neonatal (HDFn). Triplicates samples were used per each time for each gingival retraction pasts and the controls (cells treated with the serum-free medium). Plates were incubated at 37°C, 5% CO2 for the following exposure time (2 min, 10 min, and 24 hrs). One-way ANOVA and Tukey's multiple comparisons tests were performed using Graphpad Prism version 8 (Graphpad Software Inc., La Jolla, CA). The results of this study showed that there were no statistically significant differences between both experimental pastes and control paste (P> 0.05). Meanwhile, the cell viability of all experimental and control gingival retraction pastes were higher than 60% even after 24 hrs. time of exposure; therefore, both experimental gingival retraction pastes could be considered cytocompatible. However, further studies are required to test other aspects of novel gingival retraction pastes before their clinical use.
|
| Keyword:
|
Boswellia paperifera, Cytotoxicity, Astringent, MTT assay, Astringent gingival paste.
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2021.13.01.359
|
| Download:
|
Request For Article
|
|
|