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Evaluation of HER2-Neu status by immunohistochemistry and fluorescence in situ hybridization as a prognostic factor in gastric and gastro-esophageal cancer
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| Author:
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EAMAN SUUD KHALIFA, AYSER HAMEED LATIF
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| Abstract:
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Background: Amplification of the Intratumoral human epidermal growth factor receptor has been shown in a number of malignancies and few studies assess its role in gastric cancer.
Objective: This study was conducted to evaluate the clinical utility of tissue Intratumoral human epidermal growth factor receptor in gastric cancer.
Patients and methods : Clinical and pathological parameters were retrieved from medical folder in gastroenterology center/Baghdad medical city . Samples were classified according to Lauren’s Histopathological classification . Immunohistochemical staining of HER2 was manually performed using Anti ErbB2 mouse monoclonal (3b5). Out of the 74 specimens, three specimens expressing HER2-Neu was defined as IHC 2+ and subsequently was evaluated by fluorescence in situ hybridization.
Results : An immunostaining was obvious in 19 sample (25.7%). Eleven patient, (14.9%) have complete membranous ring of immunostaining in 50% to 80% of the cells (a score IHC 3+) . Three patients, (4%) had a moderate stained membrane, covering 20%, 30% and 70% of the cells (a score IHC 2+) respectively. This finding was further evaluated with fluorescence in Situ Hybridization technique looking for gene overexpression. Five patients, (6.7% of cases) have faint staining with incomplete ring seen in 30% to 50% of the cells (a score IHC 1+) . Fifty five patient (74.3%) showed no immunostaining (an IHC score 0) .
Conclusions: Perfect evaluation for HER2-Neu overexpression is essential to select patients for trastuzumab therapy.
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| Keyword:
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Gastric cancer , HER2-Neu Gene, Trastuzumab, Immunohistochemistry
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.354
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| Download:
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