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Molecular Identification of Fusarium Brachygibbosum And Some Isolates of Trichoderma Spp.
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| Author:
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ALI A.ODEH, RAJAA G.ABDALMOOHSIN, AQEEL N.AL-ABEDY
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| Abstract:
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This study was conducted to isolate and diagnose one pathogenic fungus (1), involved in root rot and seedling damping-off disease of Tomato, Solanum lycopersicum L., and four isolates of Trichoderma spp. (2, 3, 4, and 5). These isolates were molecularly identified using the polymerase chain reaction (PCR) technique and determining the nucleotide sequences of the PCR-amplified products.
PCR amplification and nucleotide sequence analysis using the Basic Local Alignment Search Tool (BLAST) demonstrated that the isolate (1) belong to the fungus Fusarium brachygibbosum giving a 100% similarity to some F. brachygibbosum isolates previously registered at the National Center for Biotechnology Information (NCBI). Results also showed that the isolate (2) belongs to the fungus Trichoderma asperellum with an identity of 100% with some other isolates already diagnosed in Australia (KU059852 and (KU059972). However, analysis of the nucleotide sequences also showed that the isolates (3 and 4) identified as Trichoderma longibrachaitum with a 96% similarity ratio between them, while they had a clear genetic variation ranged between 94-99% with the isolates previously recorded at NCBI. Moreover, identification of the isolates (5 and 6) revealed that these isolates belong to the fungus T. asperelloides with a 93% genetic variation between them and they have a 99% nucleotide sequence dissimilarity with the other the T. asperelloides isolates available in NCBI.
In conclusion, results obtained in this study demonstrated that the isolates of T. longibrachaitum (2 and 3) and T. asperelloides (6) have genetic differences with the other isolates formerly recorded in NCBI; therefore, theses isolates have been deposited under the accession numbers: MT965716, MT965717, and MT965718, respectively.
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| Keyword:
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NCBI; genetic registrations; molecular identification
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.304
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| Download:
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