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Effects of Sulforaphane in brain ischemic reperfusion injury in rats
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| Author:
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, MUNTADHER HAMEED THWAINI, AHMED R. ABU RAGHIF, NAJAH R.HADI
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| Abstract:
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Ischemic stroke is one of the major causes of mortality and disability worldwide. Limitation of cerebral blood flow due to thrombosis can cause ischemic stroke that in turn affect cellular homeostasis due to insufficient oxygen and nutrient supply. However re-establishment of cerebral blood flow can cause further deterioration of ischemic brain tissue by a series of inflammatory, apoptotic and oxidative events resulting in cerebral ischemic-reperfusion injury which in turn leads to neuronal cell death and neurological impairments. This study was carried out to evaluate the potential neuroprotective effects of sulforaphanein a global model of cerebral ischemic reperfusion injury in rats. A 24 Wistar-albino rats (weighing 200-300 grams) were divided randomly into 3 groups (n= 8 in each group), a sham (negative control) group, control (ischemic reperfusion group) and Sulforaphane group (5mg/kg) intraperitoneally. Rats were exposed to 30 minutes of ischemia followed by 1 hour of reperfusion. At the end of the reperfusion, rats were sacrificed and brain tissue samples were obtained for histopathological scoring and inflammatory markers measuring. Tissue levels of interleukin 1 beta (IL-1b), IL-6, IL-8, and tumour necrosis factor alpha (TNF-a) were significantly lower in Sulforaphane pre-treated group as compared to control group (p>0.05) in addition the histopathological score in Sulforaphane group were much lower than the control group. We concluded that administration of Sulforaphane can be useful preventive method in attenuating the degree of brain injury during ischemic reperfusion process as shown by a significant reduction of brain inflammatory markers and lower histopathological damage in comparison with control group.
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| Keyword:
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Sulforaphane, brain ischemic, rats
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.505
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| Download:
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