Cinnamon Leaf Extract to Control Anthracnose Disease on Chilli Plants in Bali: A Novel and New Potential
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Author:
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, ANAK AGUNG KETUT DARMADI, NI LUH SURIANI, IDA BAGUS GEDE DARMAYASA, IDA BAGUS MADE SUASKARA, NI MADE GARI, AHMAD FUDHOLI
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Abstract:
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Industrial synthetic fungicides, as a means of controlling plant diseases, can negatively affect the environment. Biofungicides are implemented in environmentally friendly agriculture. The implementation of biofungicides entails the use of plant extracts containing antifungal compounds to control diseases in plants. This research is conducted to determine the potential of the Cinnamomum burmannii (C. burmannii) leaf extract as a biofungicide as a means of controlling the anthracnose disease in chilli plants in Bali in vitro. The anthracnose disease is caused by the fungus Colletotrichum capsici (C. capsici). The method used in this study is the extraction of C. burmannii leaves. Then, the potential of the C. burmannii leaf extract against C. capsici with diffusion wells is tested, the minimum inhibitory concentration (MIC) is determined and scanning electron microscopy is performed. The crude extract of the C. burmannii leaves can inhibit the growth of the test fungi with an inhibition zone diameter of 2.2 cm and MIC of 0.5%. The acetone extract of the C. burmannii leaves can inhibit the growth of colonies, biomass and fungal spore germination. Some treatments, such as T2, T3 and T4, inhibit the growth of colonies in rows by 17%, 30% and 46%. At the same concentrations of T2, T3 and T4, the growth of the biomass and spores is inhibited by 14%, 24% and 87% and 51%, 69% and 86%, respectively. At the T5 and T6 treatments of the colonies, fungi biomass and spores cannot grow. The mechanism of the C. burmannii leaf extract can control fungi development by destroying the structure of fungal cell walls.
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Keyword:
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Antifungal, biofungicide, anthracnose, Colletotrichum capsici fungus
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EOI:
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DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.155
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