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Prediction of the potency of active compounds in pasak bumi root as the treatment for prostate cancer through phosphatase and tensin homolog (PTEN) pathway
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| Author:
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EKA YUDHA RAHMAN, KUSWORINI KUSWORINI, MULYOHADI ALI , BASUKI BAMBANG PURNOMO, NIA KANIA, HULDANI
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| Abstract:
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Background. Prostate cancer is one of the most commonly reported health problems in males. Prostate cancer is also the second most commonly diagnosed cancer worldwide. Currently, the incidence of prostate cancer in Indonesia is the third highest after lung and colorectal cancers. Pasak Bumi (E. longifolia jack) , from its root to its seed, are commonly used as traditional drug in that contain various active compounds. pasak bumi might have a potential as cancer suppression treatment.
Aim. To assess the potency of active compounds in pasak bumi root as prostate cancer treatment in prostate adenocarcinoma PC-3 cells through PTEN pathway.
Methods. Prediction Of Potency. The compounds in pasak bumi were searched through literature review and the SMILES (simplified molecular-input line-entry system) in the PubChem database. The potency of the compounds was analyzed with WAY2DRUG PASS prediction as anticancer.
Prediction of target. All the targets of herbal compounds were analyzed with SWISS Target Prediction in Homo sapiens. In SWISS Target, input compound was compared to other compounds in the database that have been proven to have an activity on the target protein. The more alike, the higher the similarity score. Prediction of pathway. The target predicted in stage 2 was analyzed for its interaction with target protein PTEN using the webserver STRING DB Version 11.0 in Homo sapiens model and a high confidence of 0.7 (the value that illustrate the literature supporting the results). The STITCH results provided a prediction of the pathway visualized with different colours with different meanings. Prediction score was obtained through STRING algorithm and synthesized from published study results.
Results: Based on PASS analysis, the derivative compounds in pasak bumi, including eurycomanone, have the activity as antineoplastic in prostate (Pa > 0.7) and apoptosis agonist (Pa > 0.7). The results of SWISS Target show that the compounds in pasak bumi may interact with KDR, NF?B1, and bcl2. Meanwhile, the results in PASS server show that they may interact with p53. Furthermore, they interacted with the target protein, namely, PTEN.
Conclusion: Pasak bumi can become a candidate for prostate cancer, because it increases the expression of p53. p53 has a role in DNA damage, and affects the expression of PTEN that facilitates the elimination of cancer.
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| Keyword:
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Pasak bumi root, Prostate cancer PETN
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.498
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| Download:
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