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Isolation of Chemical Marker from Fritillaria roylei: First Report
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| Author:
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, G.KAUR, V GUPTA, RG SINGHAL, MD JOSHI, P BANSAL
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| Abstract:
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Background: Fritillaria roylei (Kshirakakoli) is a threatened species of “Ashtwarga” group which suffers lot of confusion in Ayurvedic literature for identification & authentification. Due to less availability and high therapeutic value of kshirakakoli, substitution was done by drug manufacturers which in turn decrease the faith in herbal drugs. Objective: Thus for quality standardization and identification of kshirakakoli containing herbal formulations there is a need to isolate chemical marker compound using chromatographic and spectral analysis techniques. Methods: The methanol extract of root samples of plant was prepared and phytochemical screening was performed. Marker compound was isolated from extract through column chromatography. Single compound having Rf value 0.64 was isolated by TLC using mobile phase chloroform: ethyl acetate: methanol (2:3:5 v/v/v) and purified by re-crystallization with methanol. Isolated compound was characterized by using melting point and spectral analysis. Results: The methanol extract was dark brown in color and showed the presence of flavonoids, steroids and amino acids. The isolated compound was found to be white crystalline powder with melting point range of 180-185ºC. Spectral analysis confirmed the presence of Sitostanol caffeate. Conclusion: In present study sitostanol caffeate was isolated for the first time and can be used as chemical marker for identification and differentiation of the plant from substitutes.
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| Keyword:
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Kshirakakoli, Sitostanol caffeate, Isolation, Marker, Standardization.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.198
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| Download:
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