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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Evaluation of Piperine from leaves of Piper longum Linn

Author: MANISHA MOHAPATRA, UDAY C BASAK
Abstract: Piperine, the well known alkaloid, is frequently used for preparation of several drug formulations in medicament industries. It is a medicinally potent bio-molecular compound having enormous biological and pharmacological activities. Piperine is frequently extracted from the spikes and root parts of the plants of Piperaceae family. But with a little deviation to that of general fact, in this case experimentation was carried out to evaluate Piperine from leaves of Piper longum plant of Piperaceae family. Comparative assessment of extraction methods used for yielding Piperine along with their quantitative estimation through spectrophotometric and high performance liquid chromatography methods is the prior agenda. Two types of extraction processes were selected along with use of two types of solvent systems. Piperine content was estimated to be within range of 0.096-0.194% dry weight spectrophotometrically and 0.087-0.157% dry weight through HPLC analysis. From this experiment it could be opined that leaf parts of P. longum also contains piperine. This validated new result would be helpful in finding suitable alternate non-destructive source of piperine, which will lessen the threat status of the concerned species in their natural habitat.
Keyword: HPLC, Leaf, Piper longum, piperine, Spectrophotometer
DOI: https://doi.org/10.31838/ijpr/202 1.13.01.084
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0.12
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