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Production of antimicrobial compound from a novel keratinolytic bacteria Bacillus tequilensis MBR 25 for the control of foodborne pathogens
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| Author:
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BETHALA RAMYA, PANDU. BRAHMAJI RAO
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| Abstract:
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The biggest public health danger was foodborne microbial diseases. In developing and developed countries, they are built on various expansions in food production, food safety laws, food hygiene and consumption patterns, and climate and environmental conditions. After the ingestion of contaminated food, microbial pathogens are the cause of many foodborne diseases. Several methods of preservation have been developed to provide food safety for microbial food and nutritional and sensory values. Nevertheless, there is an increasing demand for microbial antimicrobial agents as a result of consumers' health concerns. The objective of this work was to isolate and characterize the bacteria from the Hyderabad, Telangana municipal dumping site. A novel potent isolate was identified by biochemical and 16s rRNA sequence methods as Bacillus tequilensis MBR 25. High activity inhibitions against foodborne bacteria and the highest 100 µg ml-1 activity levels ( E.coli 9mm, Bacillus cereus 9mm, and Staphylococcus aureus 10 mm), were observed in the extracted antimicrobial compound under the chicken feather protein hydrolysate. We have been chosen as the Aspergillus flavus for the antifungal activity based on their food grains pathogenicity. Aspergillus flavus biomass was reduced by MBR 25 culture, 60 shakings, and 50 % in static condition and extracted antimicrobial compound with a high mass reduction of 75 % on shaking, and 65% on static conditions were observed. The mode of action of the extracted antimicrobial compound against the Aspergillus flavus was relieved by scanning electron microscope (SEM) tests.
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| Keyword:
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Antimicrobial compound; Keratinolytic bacteria; Bacillus tequilensis; Foodborne pathogens; Chicken feather protein hydrolysate
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.079
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| Download:
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