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Molecular Serotyping and Genotype Variation of Escherichia coli Isolated from Urinary Tract Infections
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| Author:
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AHMED KURDI, ADNAN HAMAD AUBAID
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| Abstract:
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Urinary tract infections (UTIs) has become increasingly problematic due to the emergence of resistance to first-line antibiotics among the causative bacteria, particularly among uropathogenic Escherichia coli E. coli (UPEC) strains. So, the present study aimed to identify the genotyping of E. coli isolated from UTIs. using molecular typing. A total of 200 urine samples collected from patients definitely and clinically diagnosed with UTIs whom attended to Al-Diwaniya Teaching Hospital during the period from November 2019 to April 2020.The genomic DNA was extracted and amplified with K antigen gene cluster in E. coli UTI isolates using DNA sequencing method. The PCR product of K antigen was purified and sent to Macrogen Company in Korea for DNA sequencing and the received data was analysed using NCBI BLAST and phylogenetic tree analysis (Mega version X) between local isolates and NCBI-Blast submitted K genotypes isolates. The local isolates were submitted in NCBI-GenBank submission to get GenBank accession number. The tested human E. coli isolates showed close genetic related to NCBI-BLAST E. coli K-antigen gene cluster genotype K20 (MG739438.1) and genotype K2ab (MG736912.1) at total genetic changes (0.0020-0.030%).
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| Keyword:
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Uropathogenic E. coli; Urinary tract infections; K-antigen gene cluster; DNA sequencing.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.402
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| Download:
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