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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Nanocatalytic Efficacy of Silver Nanoparticles Fabricated Using Camellia Sinensis (Linn) Tender Leaf Extract and Their Characterization

Author: KRISHNAKUMAR S, BETHANNEY JANNEY J, SINDU DIVAKARAN, LUMEN CHRISTY V, PRAKASH WILLIAMS G
Abstract: Silver nanoparticles (Ag - NPs) was prepared by biological reduction technique. Green tea extract was assimilated as a reducing agent and stabilizing agent and silver nitrate as a metal precursor for nanoparticle synthesis. The formation of the silver nanoparticles was observed visually and UV-Visible spectroscopy. The synthesized Ag - NPs were characterized by UV-visible spectroscopy, Zeta sizer, Zeta potential, FTIR, and antibiogram studies. Silver nanoparticles were also subjected to investigate nanocatalytic activity with standard pancreatic alpha amylase and bacterial amylase enzyme by using DNS assay technique. UV-Vis. spectroscopy exposed the formation of Ag - NPs by demonstrating the typical surface resonance absorption maxima at 430 nm. Four major functional groups of bio-molecules such as phenol, carboxylic acid, protein and alkyl group were documented in FTIR spectra. The size of nanoparticles ranges between 5nm and 150nm. The average size and size distribution of silver nanoparticle is 59.66nm. The zeta potential of the Ag - NPs is negatively charged and rendered as a sharp peak at -31.7mV. Antibiogram activity of Ag - NPs exhibited highest inhibition against Gram negative bacteria than Gram positive bacteria and yeast pathogens. Starch hydrolysis using Ag-NPs was studied with pancreatic alpha amylase (tailor made), crude and purified bacterial amylase enzyme. The development of reducing sugar was increased about 40-fold when using purified enzyme, 11-fold for pancreatic enzyme and 6-fold for crude bacterial enzyme combined with Ag - NPs against control. The present studies recommended that Ag - NPs have considerable role for the degradation of complex starch into simple reducing sugar by playing as a nanocatalyst.
Keyword: silver nanoparticle; UV - visible spectroscopy; zeta potential; zeta sizer; FTIR; nanocatalyst
DOI: https://doi.org/10.31838/ijpr/2021.13.01.021
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