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A new curcumin analog, CCA-1.1, induces cell cycle arrest and senescence toward ER-positive breast cancer cells
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| Author:
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DHANIA NOVITASARI, FEBRI WULANDARI, RIRIS ISTIGHFARI JENIE, ROHMAD YUDI UTOMO, JUN-YA KATO, EDY MEIYANTO
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| Abstract:
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We proposed CCA-1.1, one of the curcumin analog derived from PGV-1 to be explored for anticancer activities toward luminal MCF-7 breast cancer cells, covering on the cytotoxic activity in correlation with cell cycle inhibition, apoptosis induction, ROS generation, and senescence evidence. The cell viability was conducted by MTT assay; cell cycle profile, apoptosis, and ROS level were measured based on the fluorochrome through flowcytometry-based assay; and senescence phenomenon was evaluated using SA-ß-gal assay. CCA-1.1 performed cytotoxic activity similarly with PGV-1 against MCF-7 cancer cells. Interestingly, CCA-1.1 induced rapid cell death and inhibited cell cycle progression particularly at G2/M, and tended to induce apoptosis. Moreover, CCA-1.1 and PGV-1 also increased intracellular ROS level, which attributed to cell senescence and led to cellular death. Taken together, our preliminary study reinforces the potency of CCA-1.1 based on its anticancer activities against luminal breast cancer cells and prospective to be pharmaceutically developed as a chemotherapeutic agent.
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| Keyword:
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Curcumin analog (CCA-1.1), cell cycle arrest, senescence, MCF-7 cancer cells
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.002
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| Download:
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