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Estimate levels of the liver enzymes and serotonin immune localization in the brain of rats administrated orally with sodium benzoate.
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| Author:
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LEENA ADEEB MEHDI, ALAA MOHAMMAD HASSON
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| Abstract:
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Sodium benzoate is used for many kinds of foodstuff; some studies reported the toxicity effects of sodium benzoate in food products. This study was conducted to observe the toxicity effects of oral administration of sodium benzoate on the liver and brain organs. Sixty male rats were used in this study, divided randomly into three equal main groups included, twenty rats. Each main groups: divided equally into two subgroups, each include ten rats: control groups received tap water while two treated main groups that administrated orally two concentrations of sodium benzoate (100 and 150 mg/kg per day) for two periods two and four weeks. At the end of each period, animals were dissected, blood samples were obtained and liver enzymes: aspartate aminotransferase AST, ALT alanine aminotransferase and alkaline phosphatase ALP were measured, samples of liver and brain were removed to histopathological changes studied and to the detection of expression of serotonin in the brain by using immunohistochemical staining technique. Results of this study proved the toxic effect of sodium benzoate as it caused an increase in the level of liver enzymes in addition to the histological change in the liver and brain tissues, also increase of immunoreactivity of serotonin in brain tissues.
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| Keyword:
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sodium benzoate, liver enzymes, brain, serotonin ,immunoreactivity.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.380
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| Download:
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