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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Phylogenetic Tree of Some Nosocomial Bacteria Isolated from Ramadi Teaching Hospital

Author: YASAMEEN HASAN ALI, AYSER ASHOUR KHALAF, FARAH JIHAD QANBAR, SAADE ABDALKAREEM JASIM
Abstract: Bacteria that resist a broad spectrum of antibiotics are known as nosocomial bacteria. Moreover, nosocomial infections cause serious problems all around the world. The aim of this study is to detect phylogenetic tree of some nosocomial bacteria isolated from Ramadi Teaching Hospital. In this study, sixty five samples were taken from different locations of the Ramadi Teaching hospital and were cultured on different media. They were also stained and examined under microscopy. It was found that there are 40 bacterial isolates and 25 fungal isolates after cultured on different media. Pure colonies were obtained after re-culture for bacterial isolates and then extracted the DNA from the 40 isolated samples and measuring its concentration. Using the specific primer for 16SrRNA genes, the samples were amplified by a PCR. All the isolated were sent to work the sequences and know the bacterial genus, as it appeared that there is a variation between the genera for different locations and also it was found that there is a variation from the comparison with the global strains of the NCBI and this indicates an evolution in some of the isolated strains and their resistance. It is important to mention that all samples isolated from the intensive care unit (ICUs), neonates, laboratories, emergency and kitchen contain variations in the sequences compared with NCBI.
Keyword: Nosocomial infections, Phylogentic tree, 16 SrRNA, Transition, Transversion.
DOI: https://doi.org/10.31838/ijpr/2020.12.04.366
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