IJPR  articles are Indexed in SCOPUS (2010 - 2021)Click Here     *Five Years Citation in Google scholar (2016 - 2020) is. 1451*        Now IJPR is Q2 in SJR Ranking     Scopus Coverage of IJPR (2010-2021 Issue 1)          Journal is Currently Not Indexed in Scopus as on february 2021 list provided by scopus

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
No Data found.
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Activity of a novel Selena-diazole derivative compound on primary neuron cells isolation from cortex and hippocampus of 18th day aged fetus of pregnant Wister rat

Author: NADHEERAH F.NEAMAH, SHAKER.A.N.AL-JADAAN
Abstract: Seleno-cysteine amino acid is originated in the active site of all selenium containing enzymes such as glutathione peroxidase. Several selenoproteins in the brain, but their roles were remained not well documented. The aim of the study is to investigate the effect of a novel selena-diazole derivative i.e. 4¯, 4¯¯ - (4, 5, 6, 7- Tetrahydro- [1, 2, 3-] selenadiazolo [4, 5e] pyridine - 4, 6 - diyl) bis (benzene-1, 3-diol) (SeD) on viability of cortical neurons and primary hippocampus cells isolated from prenatal rat pulps at 18th days. Fetal brains were removed and placed in an ice chilled Hibernated E. Hippocampi and cortices were separated. Papain 0.05% added for 10 min at 37º C; then trituration by fire polished-glass pipettes were done. Culture dishes with diameter 60 mm or 24-well plates were coated by Poly D Lysine on a density 5*104/dish. Neurons were cultured on neuro-basal medium complemented with B27 and incubated in a humidified Co2 5% atmosphere incubator at 37º C. SeD in different concentration were added, viability percentage were estimated at zero time, after 1hr, 2hr, 3hr. The results illustrated that a significant decreased in viability in a dose dependent and a time dependent manner in comparison with control and solvent groups. Despite the statistically significant level of cell viability, the levels remain high compared to other selenidiazole derivatives, also it showed the most reduction percentage after incubation for 3 hour of all treated groups, also, high significant reduction observed in 400 and 500 µg/ml treated groups. It can be concluded that SeD compound has a dose and time dependent reduce in cell viability; but still more preserve effects on neuron cells than control groups.
Keyword: Selenadiazole, primary neuron cells, viability, trypan blue, Acridine Orang Stain.
DOI: https://doi.org/10.31838/ijpr/2020.12.04.346
Download: Request For Article
 




ONLINE SUBMISSION
USER LOGIN


Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free