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Investigation and Molecular Detection of Salmonella Typhi isolated from Patients Undergoing to Gallbladder Cholecystectomy in Thi-Qar Province /Iraq
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| Author:
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ZAMAN K.HANAN, MANAL B.SALEH, EZAT H.MEZAL
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| Abstract:
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A total of (200) Gallbladder tissue specimens from Patients Undergoing to Cholecystectomy who suffering from gall bladder diseases( Cholecystitis ,Gallstones and Cancer ) were collected and diagnosed in AL-Hussein teaching Hospital and Noor Al- Hussein and Ibn-Al- Baitar laboratories) in Thi-Qar Province /Iraq during the period from December 2019 to July 2020 from both sexes and different age. According to the age the rate; age 43 - 70 years 46% is more susceptible for gall bladder diseases and According to the gender female is more susceptible than male for gall bladder diseases and were (66 %). The identification of Salmonella Typhi isolates were depended on colonial morphology and biochemical tests as a primary identification and The final identification was performed with API20 system ,the automated VITEK-2 compact system and PCR technique, twenty six (13%) clinical isolates of Salmonella Typhi were isolated and the age the rate of Patients suffering from gall bladder diseases and infected with Salmonella Typhi; age (43 - 70 years) (50%) while this study appeared female is more susceptible than male for gall bladder diseases and infected with Salmonella Typhi and were (65.4%). All the 26 isolates of Salmonella Typhi were screened for their antibiotic resistance against 16 antibiotics of different classes using the (Vitek2). The results showed that all isolates were sensitive to the Tigraracycline and levofloxacin and whereas all isolates were resistances to Ampicillin and Cefazolin; the most prevalent pattern included resistance to Gentamycin , Ciprofloxacin ,Amikacin, pipracillin/tazobactam and Cefoxitin (88,46% , 87%, 84.6% ,80.7% and 80,7% respectively ) Furthermore, many isolates were resistant to cefipime ,ceftriaxone , ceftazidime ,trimethoprim-sulphamethoxazole and Nitrofurantion (65.38% ,61.5% ,57.69% ,42.3% and 23% respectively), the resistances to Imipenem were 19.2% of isolates while only 15.38% of isolates were resistances to ertapenem, 30.7% and 23% of isolates were intermedait to Nitrofurantion and Ciprofloxacin respectively.The percentage of multidrug-resistant (MDR) bacteria was high more than (90%). All isolates of Salmonella Typhi had the ability to produce Vi antigen, which play a major role in their pathogenicity among gallbladder chronic infection.
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| Keyword:
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Gallbladder diseases, Salmonella Typhi, VITEK-2 compact system, via B gene
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.417
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| Download:
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