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Comparative Evaluation of Reduction of Microbial Count with And Without Irrigant Activation - An In Vitro Study
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| Author:
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KRISHNAKANTH JAJU, IFFAT NASIM
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| Abstract:
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Success of root canal therapy is highly predictable if proper proper cleaning and shaping protocols are followed. To achieve the root canal system free of microbes and debris root canal irrigants play a major role. The aim of the present study was to evaluate the reduction in microbial count using chlorine dioxide & sodium hypochlorite with & without activation. Forty extracted human single rooted anterior teeth were selected. The decoronated sterilized root samples were infected with 10µl of 24 hours pure culture suspension of E. faecalis for 48 hours except for 10 teeth in the negative control group (Group V). The test samples were divided into four groups (n = 10) as: Group I - 5.25% Sodium Hypochlorite with activation, Group II - 5.25% Sodium Hypochlorite without activation, Group III- 5% chlorine dioxide with activation , Group IV - 5% chlorine dioxide without activation, Group V - Negative control group. The root canals were instrumented while using respective root canal irrigant solutions. The bacterial cultures were taken from each root canal and colony forming units were counted on agar plates. The data was statistically analyzed using One Way Anova. It was observed that Group-I (5.25% Sodium Hypochlorite with activation) showed the maximum antibacterial efficacy against E. faecalis followed by Group III ( 5% chlorine dioxide with activation), Group II (5.25% Sodium Hypochlorite without activation) and Group IV ( 5% chlorine dioxide without activation). Sodium Hypochlorite combined with ultrasonic activation had the greatest antibacterial effect.
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| Keyword:
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Reduction, Comparative, Vitro Study
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.307
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| Download:
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