The effectiveness of the test plant on account of somatic mutations in leaves of soybean glycine max (l.) Merrill.
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Author:
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BITTUEVA MUKHAMATOVNA, BOGOTOVA ZALINA IKHSANOVNA, PARITOV ANZOR YURIEVICH, KHANDOKHOV TAKHIR KHAMIDBIEVICH, SABANOVA RAYA KADIROVNA
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Abstract:
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Monitoring of genetic pollutants - chemical compounds circulating in the human environment is the main task of genetic toxicology. Currently, more than 200 test systems are used to record the genotoxicity of chemical compounds and their mixtures. To assess the real pollution of the environment with potentially genetically hazardous compounds, plant test systems are most suitable. When using plant test systems, chromosomal mutations in metaphase or anaphase in meristem cells of Crepis capillaries, Vicia faba, Allium cepa, Hordeum vulgare are usually taken into account. To register gene point mutations, special lines of Tradescantia poludosa have been created and are actively used. However, the use of these tests allows taking into account only one type of mutational events, either chromosomal mutations (aberrations) or gene mutations.
The greatest advantage in this regard is the test for accounting for somatic mutations in the leaves of soybean Glycine max. L. Merrill, which allows differentiated registration of several genetic DNA lesions at different levels at once, namely, direct and reverse gene mutations, induced somatic crossing over and chromosome nondisjunction. This advantage, as well as the speed and cost-effectiveness of research, make this test system very promising when analyzing the genetic danger of pollution of territories and industries.
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Keyword:
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plant test systems, Soybean Glycine max L. (Merill) line ?219, mutagenic activity of chemical compounds
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EOI:
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-
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DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.318
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