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An in silico approach to identify potential protein targets of rutin in common dental pathogens
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| Author:
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R.JAGADISH RAJKUMAAR, SMILINE GIRIJA A.S, J.VIJAYASHREE PRIYADHARSINI
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| Abstract:
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To demonstrate the antimicrobial activity of rutin against common dental pathogens, we used in silico approach in the present study. Medicinal plants and flowers are the most common components of traditional medication. Drug discovery has witnessed phytochemicals for the latest alternatives to chemical drugs. Flavonoids, polyphenolic compounds are the critical training of plant derived chemicals that comprise benzopyrone group.The present study has been designed to analyse and identify potential antimicrobial activity of of the phytocompound rutin to common dental pathogens. Computational tools were used to identify the targets of rutin and assess its functional role and virulence property. The subcellular location of the virulence proteins were also elucidated using PSORTb. Proteins interacting with Rutin were primarily related to metabolism, followed by cellular processes and information storage. Interestingly, the scores from VirulentPred marked citrate synthase to act in the cellular process to be virulent positive with a score of about 0.431. And the rest of the proteins analyzed were found to be avirulent, STITCH prediction for Rutin returned proteins mainly associated with metabolic and cellular processes. Mercury(II) reductase, involved in cell’s metabolism, was found to be the virulent factor with a positive virulent score of about 0.689. In the present study, it was found that rutin demonstrated a significant virulence activity against S.mutants and Fusobacterium nucleatum, Streptococcus mutans is the most common bacteria which is involved in causing dental caries so the virulence factor exhibited by rutin against Streptococcus mutans can be utilized to reduce the caries prevalence among the highly caries susceptible individuals.
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| Keyword:
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Antimicrobial activity; in silico; Rutin; Phytocompound; Virulence
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.323
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| Download:
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