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An in-silico study investigating the differential gene expression of Rho family GTPases and their putative association with HNSCC
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| Author:
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AKASH.N , DR.SMILINE GIRIJA A.S, , DR.A.PARAMASIVAM, DR.J.VIJAYASHREE PRIYADHARSINI
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| Abstract:
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Rho GTPases are master regulators of actomyosin structure and dynamics and play pivotal roles in a variety of cellular processes including cell morphology, gene transcription, cell cycle progression and cell adhesion. The present study follows an observational study design employing computational tools. The source of patient’s data was procured from the cBioportal database. This database hosts an exhaustive collection of patient’s details from different cohorts. The aberrant expression of Rho GTPase is widely associated with human cancer, which is due to deletion/ addition or amplification of the gene. Gene expression profile and survival analysis was carried out using - UALCAN database. Gene alterations in the Rho GTPase family were identified using - cBioportal. Among 20 genes of the Rho family, five genes RAC2, RAC3, RHOF, RHOG and RHOH were found to exhibit a differential expression pattern with a statistically significant value less than 10-12. The effect of differential gene expression RHOG with different parameters such as grade, gender and race produced statistically significant value less than 0.05. Although, the gene alterations identified in Rho GTPase family genes did not correlate with the oncoprint data, which showed a maximum of 7% alteration in the RHOD gene, the expression pattern of other members of the family in HNSCC patients as documented in the study warrants further investigations to be carried out in these genes.
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| Keyword:
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: Rho family, GTPases; Squamous cell carcinomas; actinomycin; cancer.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.314
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| Download:
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