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Evaluation of Sterility of Newly Opened Endodontic Hand Files
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| Author:
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C.S YUVASHREE, ANJANEYULU K
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| Abstract:
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Infection control and sterilization plays an very important role in dentistry because microorganisms are the major cause for several endodontic diseases . Endodontic treatment involves various working procedures, such as the isolation of the operating field, pulp chamber access, and cleaning and shaping phases in the canals and last the filling of a three-dimensional canals. Each step requires a set of sterilizable instruments.The newly obtained files may not necessarily be guaranteed as sterile. Thus the aim of the study was to ascertain the sterility of new endodontic hand files received from different manufacturing companies. Twenty endodontic hand files from two different manufacturers namely, Mani and Dentsply were taken . 10 files files from each manufacturer. Each file was transferred to an eppendorf tube containing 3 ml of sterilized Brain Heart Infusion (BHI) under sterile conditions. Additionally, twenty eppendorf tubes with 3 ml of sterilized Brain Heart Infusion broth without the files were taken as control. The test tubes were incubated as static cultures at 37°C for 72 hours and color change indicating the presence of bacterial growth were documented. Statistical analysis was done on SPSS software version 2.0 by IBM and the chi square test was done to assess the significance of the study.The study shows that 25% of the endodontic hand files received from the manufacturer were biologically contaminated. Within the limitations of the study, it is established that most endodontic files received from the manufacturer have the potential to be contaminated with viable microorganisms and that proper sterilization of the files before clinical use is mandatory
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| Keyword:
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H- file, k file, Endodontic hand files, bacteria, sterility.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.313
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| Download:
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Request For Article
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