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Virtual screening to identify virulence factors in red complex pathogens targeted by naringin.
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| Author:
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LASYA GENJI, SMILINE GIRIJA A.S, VIJAYASHREE PRIYADHARSINI.J
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| Abstract:
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Red complex pathogens are associated with periodontal and deep seated oro-dental infections. Eradication of these pathogens seems to be a herculean task due to several factors including biofilm formation, drug resistance etc., The present investigation is aimed to evaluate the functional and molecular virulent class proteins to be targeted by the phyto-compound naringin against the group of red complex dental pathogens using in-silico tools. The study design intended to detect virulence proteins of the dental pathogens too. An in-silico study design is performed to identify the potential virulence factors targeted by naringin. Drug-protein interaction was detected by STITCH tool followed by the prediction of the functional class and virulence encoded proteins by VICMPred and VirulentPred tools respectively. Further, peptide epitopes of the virulent proteins were evaluated by PSORTb and BepiPred tools. Naringin targeted most of the proteins associated with cellular metabolism. Only one conserved hypothetical virulent protein was predicted from T.denticola. Naringin showed maximum protein interactions with T.denticola, with glutathione peroxidase as the commonest target among T.denticola and T.forsythia. The findings of the study emphasize the promising interactive role of naringin in combating the infections with the common red complex dental pathogens with further experimental validation in-vivo and in-vitro.
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| Keyword:
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Naringin, STITCH tool, virulence, epitopes, antimicrobial
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.281
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| Download:
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