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Serological and molecular detection of Epstein –Barr virus (EBV) in patients with malignant lymphoid solids
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| Author:
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SALWA S.SHIHAB, HAYDER ABDULHUSSEIN AL-HMUDI, SADEQ K.ALI.AL.SALAIT
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| Abstract:
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Background: Epstein-Barr virus (EBV) is a common herpesvirus can be associated with numerous infections and cancers. This study aimed to diagnosis of EBV among malignant lymphoid solids of patients in Basrah province/Iraq.
Patients and Methods: A 120 blood samples of both sexes with the age range 6 months to 93 years’ old were collected from patients with malignant lymphoid solids. All serum samples were examined to detect EBV biomarkers including (anti-EBV-CA (IgG), anti-EBV-CA (IgM) and anti-EBV-NA-(IgG) antibodies. The nested PCR protocol was used to amplify two DNA fragment of EBV.
Results: The results of patients showed positive sera of 14/120(11.66%), 120/120(100%) and 111/120(92.5%) of anti-EBV capsid antigen IgM, anti-EBV capsid antigen IgG and anti-EBV nuclear antigen IgG, respectively. The present study was showed 4 statuses (Table, 1) among studied groups; a 99/120 (82.50%), 12/120 (10.0%), 7/120(5.83%) and 2/120(1.66%) patients as seropositive infection, reactivated infection, old infection and recent primary, respectively. EBV-DNA was detected in 44/120(36.7%) and 35/100(35%) in patients and control groups, respectively.
Conclusions: we concluded that highly seroprevalence of EBV among malignant lymphoid solids patients.
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| Keyword:
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Epstein-Barr virus, Acute lymphoblastic leukaemia, Hodgkin lymphoma.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.306
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| Download:
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