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Molecular detection of human Parvovirus B19 in patients with hemoglobinopathies in Basrah province-Iraq
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| Author:
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MOHANED A.K.AL.ATBEE, HAYDER A.M.AL.HMUDI, SADEQ K.A.AL.SALAIT
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| Abstract:
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Background: Human Parvovirus B19 infects human and cause no or mild disease, but more severe disease may occur in some people especially those with thalassemia and sickle cell anemia. In HPV-B19 infections lead to suppression of the erythrocytes formation and acute erythroblastopenia often called transient aplasia crisis which may be life-threatening. The aim of this study was to detect the prevalence of Human parvovirus B19 in thalassemia and sickle cell patients in Basrah provance, Iraq molecularly.
Patients and Methods: A 208 serum samples of both sexes with the age range 1 to 47 years’ old were collected from patients with haemoglobinopathies. The nested PCR protocol was used to amplify two DNA fragment of B19. The PCR products of the second round (288 bp) and a 926 bp DNA fragment spanning the NS1-VP1u junction were sent for sequencing.
Results: Human parvovirus B19 was detected in 42/208(20.2%) and 15/100(15%) of patients and control groups respectively. The studied patients were divided into 10 age groups, the age periods (1-5) and (10-15) were significantly prone to the infection (P< 0.05). In contrast, the age period (46-50) years had significantly lower infection (P< 0.05). Also, the patients were divided into 5 study groups. Sequencing of DNA for confirming virus identification showed that of 57 PCR products, 26 isolates were successfully sequenced.
Conclusions: we concluded that low prevalence of HPV-B19 in thalassemia and sickle cell patients.
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| Keyword:
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Human Parvovirus B19, Thalassemia, sickle cell, Polymerase Chain Reaction.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.305
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| Download:
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