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Genetic Detection of Pseudomonas spp. and Microbial Quality Assessment in Marine Fish are Available in Local Markets of Baghdad City
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| Author:
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ALIAA S.A.AL-FRAJI, SUDAD JASIM MOHAMMED, ADIL.T. AL – MOUSAWI
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| Abstract:
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Pseudomonas spp. present everywhere around the world and isolated from water, domestic animals, marine fish, human being then additionally from a range of nutrition. This study was conducted to isolated and detects Pseudomonas spp. in frozen marine fish obtained from local market of Baghdad City, IRAQ. Fifteen samples of frozen marine fish were randomly collected from Baghdad City. Twenty five gram of each specimen are treated with 225 ml of peptone water, incubated at 37C for 24 hr. Samples were streaked on specific culture media. Afterward inside laboratory, polymerase chain reaction methods are involved to find Pseudomonas spp. All experimental fish are contaminated with Pseudomonas spp. M15 (31×104) CFU/g. and M4 (7×104) CFU/g respectively. While result of PCR techniques were confirmed diagnosis of Pseudomonas aeruginosa (bp 600).
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| Keyword:
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Genetic Detection, Marine Fish, Pseudomonas spp.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.275
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| Download:
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