IJPR  articles are Indexed in SCOPUS (2010 - 2021)Click Here     *Five Years Citation in Google scholar (2016 - 2020) is. 1451*        Now IJPR is Q2 in SJR Ranking     Scopus Coverage of IJPR (2010-2021 Issue 1)          Journal is Currently Not Indexed in Scopus as on february 2021 list provided by scopus

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
No Data found.
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Extraction and Purification of ß-Galactosidase from Lettuce (Lactuca sativa L.)

Author: SARI HUSSIEN, ABDULHAKEEM DAHHAM HUSSEIN, OTHMAN GHAZI NAJEEB
Abstract: ß-galactosidase enzyme EC (3.2.1.23), lactase, can be described as an enzyme of glycoside hydrolase which performs the catalyzing of ß-galactosides hydrolysis to mono-saccharides by breaking glycosidic bond. The objective of this study was to extraction and purification ß-galactosidase from lettuce (Lactuca sativa L.), ten Different types of extraction were investigated to selection of the best extraction of the enzyme, The Na. phosphate buffer (0.2M and pH6) had given a highest Specific activity of crude enzyme has been 151.09 U/mg. protein. The purification procedures were performed with the use of the precipitation of ammonium sulfate, ion-exchange and gel filtration chromatographic techniques. 70% ammonium sulfate saturation has been the best method for precipitation and partially purification of enzyme with a purification fold 1.54 and enzymatic yield 60.6%. This was followed by the use of ion exchange chromatography by DEAE Sephadex A50 column, the purification times of the enzymatic extract were 3.18, with an enzymatic yield 21.33%. After the final purification step of gel filtration chromatography using SephadexG-100 column, the enzyme has been purified 4.84 fold with 13.68% of enzymatic yield. The optimum enzymatic activity was found at pH 6. The enzyme’s molecular weight has been estimated to 79.43 KD by the gel filtration chromatography method, and 78.93 kD on SDS-PAGE.
Keyword: Lettuce, ß-Galactosidase, Extraction, Purification Methods.
DOI: https://doi.org/10.31838/ijpr/2020.12.04.274
Download: Request For Article
 




ONLINE SUBMISSION
USER LOGIN


Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free