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Comparative Diagnostic Value of Anti-Dengue IgG, Anti-Dengue IgM of Two Rapid Tests in Dengue Virus Infection
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| Author:
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MAY TANZILIA, NELLY ZUROIDAH, I GUSTI AGUNG AYU EKA PUTRI SUNARI, BILLY JORDAN WRAHATNALA, FARADILA KHOIRUN NISA, HAKIM, ALI ROHMAN, PUSPA WARDHANI, DOMINICUS HUSADA, SITI NADIA TARMIZI, ARYATI
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| Abstract:
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Introduction: Diagnosis of Dengue Virus Infection (DVI) is based on combination of clinical manifestation, hematological, serological, and molecular examination. This study aims to compare the diagnostic value of anti-dengue IgG and anti-dengue IgM by two rapid tests with RT-PCR Dengue and ELISA as the gold standards.
Methods: This was an observational cross-sectional study. Serum was collected from 80 febrile patients (3-7 day fever), who were suspected Dengue Virus Infection (DVI). Serum was examined by STANDARD Q-SD and SD BIOLINE as rapid tests and RT-PCR and or NS1 Ag Dengue (ELISA) and anti-dengue IgG/IgM ELISA as the gold standards.
Results: The most common serotype identification was DENV 3. The positive results of NS1 Ag Dengue were common on day 3 of fever in primary infection and day 4 of fever in secondary infection. The antibody examination was dominated by positive anti-dengue IgM and positive anti-dengue IgG in 27 patients (54%) in secondary infection. Sensitivity anti-dengue IgG STANDARD Q was 68%, and SD BIOLINE was 87%. Specificity anti-dengue IgG STANDARD Q was 80%, and SD BIOLINE was 76.7%. Sensitivity IgM anti-dengue STANDARD Q was 44%, and SD BIOLINE was 48%. Specificity anti-dengue IgM of two rapid tests was 100%.
Conclusion: The serological examination with rapid tests is not enough to establish a diagnosis of DVI, so the combination with NS1 Ag Dengue and RT-PCR examination is needed.
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| Keyword:
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Dengue virus, diagnosis, rapid test, ELISA
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.241
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