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The Association Between Genotypes And Clinical Symptoms Of Giardia Lamblia In Patients With Symptomatic Giardiasis
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SUNDUS AL-HUCHAIMI, MOHAMMED KAMIL AL-HASSANI, ABDULKAREEM KHATTAR SHAALAN ALHATEMI, MAJIDA MALIK METEAB ALSHAMMARI, THIKRA A.MAHMOOD
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| Abstract:
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Giardia lamblia is most common humans intestinal pathogen worldwide. The current study is performed to detect the association between genotype and disease outcome .The parasite isolated from human faeces in Najaf province were analyzed with nested PCR and PCR-restriction fragment length polymorphism (RFLP) assay, based on the detection of triose phosphate isomerase (TPI) gene. Among 100 stool samples from microscopically diagnosed giardiasis patients, the TPI gene was amplified from 75 cases with a semi nested PCR assay and typed by RFLP analysis. Of the 75 positive samples, 22 (29.3%) were typed as assemblage A, while assemblage B were recorded 53(70.6 %).Restriction fragment length polymorphism(RFLP) revealed that 4 (5.3%) and18 (24%) of the assemblage A were A? and A? respectively, also showed 43 ( 57.3%) of assemblage B to be BIII and 10 (13.3%) to be BIV. Clinical features were available for 75 successfully typed cases and the possible correlation of Giardia assemblages and clinical symptoms was evaluated. Both assemblages caused similar illness, but assemblage AII was significantly more frequently associated with diarhea, vomiting and flatulence .There is association between clinical symptoms and genetic variation of the parasite, therefore further studies are required to wider investigations .
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| Keyword:
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Giardia lamblia, genotype, clinical symptoms ,Iraq
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.239
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