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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Effect of SIRT1 Activators on Human Follicular Thyroid Cancer

Author: LAMEES TALIB, MUSTAFA GHAZI AL-ABBASSI, BASMA TALIB AL-SUDANI
Abstract: Sirtuins are NAD+ dependent deacetylase enzymes, SIRT1 is the most studied type of human sirtuins. SIRT1 plays a dual role in cancer where it can be a tumor promoter or tumor suppressor. The activation of SIRT1 by aptamer shows a tumor-suppressive effect in multiple cancer cell lines. This study aims to study the effect of activation of SIRT1 by aptamer on FTC236 and FTC238 by studying the mechanism of p53 and MAPK. The methodology includes circularization of aptamer, cell line culture, MTT assay and determination of IC50, cell fractionation, immunoblotting, transfection of p53 antisense oligonucleotides, and determination of c-fos, p21, and GAPDH mRNA. The result shows that the activation of SIRT1 by aptamer could inhibit significantly the growth of FTC236 and FTC238 cell lines, this inhibition occurs through the activation of MAPK (ERK1/ERK2) and subsequent expression of p21 and c-fos.
Keyword: MAPK (ERK1/ERK2), p21, c-fos, MTT assay.
DOI: https://doi.org/10.31838/ijpr/2020.12.04.230
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