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In vitro cytotoxicity of Dragon Fruit Extract (Hylocereus polyrhizus) on Fibroblast Cell Culture
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| Author:
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WISNU JULIASTUTI, HENDRIK SETIA BUDI, DINDA AKHLAKUL KARIMAH
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| Abstract:
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The wound from tooth extraction must be handled as soon as possible to prevent any complication, such as: overwhelming pain, bleeding, inflammation, disturbance on mastication function and speech function and causing dangerous infection; to prevent those complications, a medication is administered to help accelerate healing process. These days, there are the development of the usage of natural ingredient as the alternative medication, one of those medications is dragonfruit rind (Hylocereus polyrhizus), because the dragonfruit rind has many benefits, such as antioxidant and antibacterial. But the safety of the dragonfruit rind as alternative medication must be proven through cytotoxicity test by using fibroblast cell culture of Baby Hamster Kidney-21 (BHK-21). The aims of this study to determine the toxicity of the extract of the dragonfruit rind (Hylocereus polyrhizus) towards fibroblast cell. The study is laboratory experiment by measuring the level of optical density of fibroblast cell using Methyltiazolyldiphenyl-tetrazolium bromide (MTT) assay. The fibroblast cell culture of BHK-21/C13 is treated with the extract of the dragonfruit rind with the concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%. The bigger the percentage of the extract of the dragonfruit rind, the level of optical density becomes smaller. It shows that the higher the concentration of the medication, the smaller the number of cells that survive and 30% concentration is the optimal concentration to have =60% of surviving cells. The extract of the dragonfruit rind (Hylocereus polyrhizus) shows toxic effect towards fibroblast cell of BHK-21 from 60% concentration.
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| Keyword:
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The dragonfruit rind (Hylocereus polyrhizus), MTT assay, Fibroblast cell of BHK-21
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.195
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| Download:
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