IJPR  articles are Indexed in SCOPUS (2010 - 2021)Click Here     *Five Years Citation in Google scholar (2016 - 2020) is. 1451*        Now IJPR is Q2 in SJR Ranking     Scopus Coverage of IJPR (2010-2021 Issue 1)          Journal is Currently Not Indexed in Scopus as on february 2021 list provided by scopus

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
No Data found.
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Investigation of Antibacterial Effect and Expression of Alkaline Protease Gene in Pseudomonas Aeruginosa (PAO1) Using Copper Oxide, Copper Complex and Satureja Khuzistanica: RT-PCR Method

Author: RAZIYEH MOHSENI, MEHRI HOSSEINI, MEHRORANG GHAEDI, ASGHAR SHARIFI, HAMID REZA MOHAMMADI, FOROUGH MORIDIKIA, HASSAN ABIDI, DAVOUD ESMAEILI, SEYED REZA KHOSRAVANI, SEYED ABDOLMAJID KHOSRAVANI
Abstract: Background and objective: In the genus Pseudomondaceae, exists Pseudomonas aeruginosa which is a gram-negative bacterium. It is an opportunistic human pathogen that frequently colonizes patients who are immunocompromised, such as those with cystic fibrosis, cancer or AIDS. Pseudomonas aeruginosa also has inherent resistance to several different chemotherapeutic agents and antibiotics. This study intended to evaluate the inhibition effect of copper oxide nanoparticle (CuO), copper nano complex (Cu complex) and the Satureja Khuzistanica essence on the gene of alkaline protease in P. aeruginosa (PAO1) by RT-PCR method. Method and material: Satureja Khuzistanica essence was synthesized by Clevenger apparatus. Nanoparticular size and morphology were characterized by the use of SEM. The antimicrobial activity of these substances was calculated from the process of disk diffusion and microbroth dilution based on CLSI norms. After determination of the Minimum Inhibitory Concentration (MIC) and Zone of Inhibition (ZOI), these data were analyzed using SPSS 21 software, through descriptive and inferential statistics at a 95% confidence level. In this study, ethical considerations were respected. Results: Zone of inhibition (ZOI) and Minimum inhibitory concentration (MIC) of Satureja Khuzistanica essence, CuO and Cu complex for PAO1 were found to be 19, 15 and 9 mm and 4, 12.5, and 25µg/ml respectively. Furthermore, the MIC synergism of Satureja Khuzistanica essence and CuO was 2 and 3.125µg/ml and Satureja Khuzistanica essence and Cu complex was 2 and 6.25µg/ml respectively. The ZOI synergism of Satureja Khuzistanica essence and CuO was 23mm and Satureja Khuzistanica essence and Cu complex were reported 20mm. Conclusion: According to the results obtained from the present study, a significant antibacterial activity was seen by Satureja Khuzistanica essence. By examining the synergism effect of CuO, Satureja khuzistanica essence, and Cu complex essence simultaneously, the further antimicrobial effect than was observed when each were used alone.
Keyword: CuO, Cu complex, bacterium, chemotherapeutic agents
DOI: https://doi.org/10.31838/ijpr/2020.12.04.067
Download: Request For Article
 




ONLINE SUBMISSION
USER LOGIN


Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free