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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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In vitro study of antibacterial and antioxidant properties of urine of indigenous Badri cow

Author: VIPIN NAUTIYAL, R.C. DUBEY
Abstract: Cow urine occupies a holy place in Indian rituals and Ayurveda due to its effective application against various diseases since Vedic period. The present study was carried out to evaluate the antibacterial and antioxidant properties of Badri cow urine. The antibacterial potential was evaluated against Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella typhi and minimum inhibitory concentration (MIC) was evaluated following different methods. The total phenolic content, free radical and superoxide radical scavenging activity were assessed by FC, DPPH and NBT method. Cow urine inhibited the growth of all selected bacterial pathogens with different potency; however, S. aureus, L. monocytogenes and E. coli were the most susceptible bacterial strains to cow urine even at low concentration. Total phenolic content of cow urine was 5.09 mg GAE/g. The DPPH and NBT assays showed dose-dependent scavenging activity of cow urine. Due to presence of both antibacterial and antioxidant properties cow urine may be used as a therapeutic agent.
Keyword: Badri cow urine, Antibacterial activity, DPPH, NBT
DOI: https://doi.org/10.31838/ijpr/2020.12.04.077
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0.12
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