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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Anticancer Properties of Safawi Date (Phoenix Dactylifera L.) Seed Extract against Human Breast Adenocarcinoma Cell Line (MCF-7): In-vitro Study

Author: NURUL AHMAD, ROZILA ISMAIL , LAW JIA XIAN
Abstract: Breast cancer is the most common cancer in women in most parts of the world. It is well-known that conventional treatment for breast cancer is associated with significant adverse effects, high mortality rate and increased cost of treatment. Thus, the search for natural products for cancer therapy is an area of great interest. The aim of the study was to investigate the anticancer properties of methanolic extract of Safawi date seed (MESDS) against human breast adenocarcinoma (MCF-7) cells. Firstly, the cytotoxic activity was determined via CellTiter®-Blue cell viability assay which revealed that the two highest MESDS concentration used in the study (1 mg/mL and 10 mg/mL) exhibited significant reduction in MCF-7 cell viability when compared to the positive control group (p=0.003 and p<0.001 respectively) for 72 hours treatment. However, there was no significant difference in cell viability reduction against MCF-7 cells treated with MESDS for 24 hours. The calculated IC50 values were 10.62 mg/mL for 24 hours and 1.22 mg/mL for 72 hours which are categorized as cytotoxic. Next, cytological observations by hoechst 33342/propidium iodide dual-staining assay showed typical apoptotic morphology of cancer cells upon incubation with 10 mg/mL MESDS for 24 and 72 hours. Finally, the anti-migration activity of MESDS was evaluated using scratch assay. Treatment with 10 mg/mL of MESDS significantly decreased MCF-7 cell migration when compared to the untreated control group. Collectively, date seeds are seen as potential anticancer agents following their abilities to reduce viability, induce apoptosis, and suppress migration of MCF-7 cells.
Keyword: Date Seed, Anticancer, Apoptosis.
DOI: https://doi.org/10.31838/ijpr/2020.12.03.289
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