The relationship of the adhesion of the oral cavity microorganisms with the finishing method of dental material restoration
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Author:
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K.A. SARYCHEVA, S.V. KAMENEVA, N.V. KUSHCHENKO, L.A. KRAEVA, D.S. USIKOVA
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Abstract:
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This research is aimed at analyzing the interaction of microorganisms of the oral cavity with dental filling materials. It was suggested that the adhesion of microbiota representatives to the restoration surface depends on the method of its finishing. The work is aimed to study the colonization of bacteria on nanocomposite filling material (Filtek Ultimate Enamel A1) depending on the method of its final processing in vitro. The studied microorganisms: Streptococcus salivarius - a caries pathogenic representative of the constant microflora of the oral cavity, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecium - periodontopathogenic representatives of the unstable microflora of the upper respiratory tract.
The prepared samples of the restoration material differed like their surface: perfectly even, treated with a finishing diamond bur and with a surface inhibited by oxygen. During bacteriological research, we carried out and estimated the luminescence microscopy of colonies formed by microorganisms.
According to the results of the experiment, the following conclusions were made: 1) bacteria adheres to the filling material even in the absence of retention points and voids on its surface; 2) on ideally even samples, in comparison with other surfaces, the number of bacteria colonies of E. faecium is higher; on the surfaces treated with boron - K. pneumoniae; and on samples with an oxygen inhibited layer - P. aeruginosa.
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Keyword:
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bacterial adhesion, dental plaque, nanocomposite material, finishing method
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EOI:
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-
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DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.112
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