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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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DNA Sequencing of blaSHV Genes among Uropathogenic Klebsiella pneumoniae Harboring blaCTX-M-15

Author: SARA MOHAMED, MARY S. KHALIL
Abstract: Introduction: The spread of antimicrobial resistance in ESBLs Klebsiella pneumoniae strains usually makes nosocomial infection treatment difficult. Mostly, ESBLs found in this pathogens carry the TEM and SHV, this study was designed to detect the SHV variant among two K. pneumoniae isolates harboring blaCTX-M-15. Materials and methods: Two uropathogenic Klebsiella pneumoniae strains were identified, and by using the recommended methods cephalosporins susceptibility was detected. Universal primer for blaSHV was used, and then products were subjected to sequencing. Results: The two isolates showed highly resistant to all tested cephalosporins different generations, at which the MIC results for cefradine, cefuroxime and cefixime were ? 128 µg/ml, 128 µg/ml, 32 µg/ml and ? 128 µg/ml, ? 128 µg/ml, ? 128 µg/ml for strains K42 and Kp7 respectively. The sequencing of amplicons from the blaSHV group showed high identity with different known blaSHV like variants. Conclusion: The occurrence of blaSHV like genes among two Egyptian ESBL producing Klebsiella pneumoniae strains was demonstrated in this study. High similarity with different other global genes in the gene bank documented in NCBI was obtained. Therefore, further studies on a large blaSHV carrier of Klebsiella pneumoniae are in need to detect the prevalence of these genes.
Keyword: blaSHV; ESBL; Klebsiella pneumoniae; Uro-pathogen
DOI: https://doi.org/10.31838/ijpr/2020.12.04.057
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