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Optimization of etodolac loaded emulsomes by box-behnken design of experiment
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| Author:
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VIVEK GILL, ARUN NANDA
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| Abstract:
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Objective:
The present work is focused on the optimization of Emulsomes formulation by applying Box-Behnken design (BBD) of experiment. Emulsomes are the modified form of liposomes which consists of solid lipid core surrounded by phospholipid bilayer. BBD is response surface type design of experiment.
Method:
Emulsomes were prepared by lipid film hydration method using Phosphatidylcholine (PHL), Tristearin (TRI), Stearylamine (STR), Cholesterol (CHOL) and Etodolac as drug candidate. Formulations were optimized by using three factor three levels Box-Behnken (BBD) design of experiment. BBD was applied considering 3 factors as independent variables viz. Drugs to Phospholipid ratio (A), Tristearin to Phospholipid ratio (B) and Stearylamine to Phospholipid ratio (C) at 3 levels (-1, 0, +1) to study their effect on dependent variables viz. Particle size (Y1), Zeta Potential (Y2) and Entrapment efficiency (Y3). Results of independent variables (A,B,C) were analysed to construct quadratic equation and 3-D response surface curves for each dependent variable (Y1,Y2,Y3).
Results:
The optimized formulation was selected on the basis of desirability criteria of minimum particle size, maximum zeta potential and maximum entrapment efficiency. The final optimized formulation have predicted particle size (390.394 nm), zeta potential (45.0 mV) and entrapment efficiency (81.642 %).
Conclusion:
BBD is significant design of experiment for studying the effect of 3-factors at 3-levels on the responses in optimizing the emulsomes formulation.
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| Keyword:
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Box-Behnken Design (BBD), Emulsomes, Etodolac, Phospholipid, Stearylamine, Tristearin, Variable.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.056
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| Download:
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