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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Molecular Screening of Virulence Genes among Uropathogenic Enterococcus faecalis Isolated from patients in Al-Najaf City, Iraq

Abstract: Background: Enterococcus faecalis is a common nosocomial pathogen that is a dominant causative agent for urinary tract infection (UTIs) of humans in the community. Materials and Methods: Out of 670 urine specimens were obtained from patients recurred to the main hospitals (Al-Hakeem General, Al-Furat Al-Awsat, and Al-Sadr Medical City), and some chief clinical laboratories in Al-Najaf City. Results: The E. faecalis isolates were recognized based on commercial standard techniques, ChromaticTM Detection, Vitek-2 compact system, and PCR using SodA gene, the result exposed that 21(3.134%) isolates were returned to E. faecalis. The data of current work was revealed out of 670 urine specimens were recorded 452(67.462%) bacterial growth included 142(21.194%) gram-positive and 310(46.268%) was gram-negative while 218(32.537%) was no bacterial growth. The data observed the frequency of urinary tract infections was found to be most in female 507(75.671%) than male163 (24.328%). Furthermore, the rate of E.faecalis isolates in females was 19(90.476%) compared with 2(9.523%) in males. The antibacterial agents' susceptibility of E. faecalis revealed different rates of resistance starting with high rate 21/21(100%) and 20/21(95.238%) of isolates which were resistant to ampicillin and erythromycin respectively, then terminating with lower resistance among these uropathogenic isolates which were 2/21(9.523%) and 3(14.285%) to chloramphenicol and doxycycline respectively. Molecular screening about some virulence factors genes involving asa-1, cyl-A, ebp, pai, and SprE genes were observed at different rates reach 80.952%, 90.476%, 95.238%, 23.809%, and 90.476% respectively. Furthermore, the rate of gyrB and Int-1genes were 95.238% among E. faecalis isolates, while 76.190% of the isolates were harbored blaZ gene. GyrA and tetA genes were not found in the present work. Conclusion: E. faecalis isolates were a significant causative among gram-positive pathogens that generate UTIs to humans. Elevation rates of Multi-drug resistance among isolates of E. faecalis, as well as there, was no drug was absolute inhibition to all isolates in this work and this is a concern source. E. faecalis isolates were harbored most virulence element exception tetA and gyrA were not detected in this work.
Keyword: Enterococcus faecalis , antibacterial resistance, virulence factors, UTIs
DOI: https://doi.org/10.31838/ijpr/2020.12.04.051
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