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Investigation phytochemical screening, antibacterial activity, and cytotoxic ethanolic extract of Geodorum densiflorum (lam.) Schltr from papua
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| Author:
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ELSYE GUNAWAN, VERENA AGUSTINI, EVA SUSANTY SIMAREMARE
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| Abstract:
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One of ten species of orchid plant (genus Geodorum) that widespread in tropical forests of Asia including Indonesia (Papua) is Geodorum densiflorum. G. densiflorum showed significant pharmacological activities such as antibacterials, cytotoxic, antioxidant, antifungal, thrombolytic, and analgesic. So this study aims were to determine the active compounds, antibacterial activity, and cytotoxicity extracts and fractions of G. densiflorum coming from Papua.
Samples (leaves and tubers) of G. densiflorum were made in simplicia, extracted with ethanol and fractionated using n-hexane, ethyl acetate, and ethanol solvent. Extract as well as fraction were tested for phytochemical screening then determined antibacterial activity against Staphylococcus aureus bacteria by Disc Diffusion Method. In spite of cytotoxic test was used Brine Shrimp Lethality Test (BSLT) method with variation of extract and fraction 10, 50, 100, 250, 500, 750, and 1000 ppm.
The results showed that the extract G. densiflorum positive contained tannin and alkaloids in leaves futhermore flavanoids and alkaloid in tubers. G. densiforum has activity inhibiting the growth of S. aureus bacteria. The zone of inhibition extract were in [8 mm (leaves), 8 mm (tuber)] and maximum fraction were in [n-hexane solvent was 8 mm (leaf) and ethanol was 7 mm (tuber)]. The cytotoxic test showed that LC50 extract were in [1281.4 ppm (leaf) and 1640.9 ppm (tuber)] during the maximum LC50 the fraction of n-hexane were in [739.2 ppm (leaf) and 785 ppm (tuber)]
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| Keyword:
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G. densiflorum, phytochemical screening, antibacterial, cytotoxicity, Papua
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.048
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| Download:
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