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Detection of Heat Sock Protein 70 gene in Gardia lamblia in Thi-Qar province.
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| Author:
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SAJA YASIR, FADIL ABBAS MANSHUD AL-ABADY, HAIDER MOHAMMED FAYYAD ALYASIRI
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| Abstract:
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Background: Thermal shock proteins (HSPs) are a group of highly preserved proteins found in all organisms that created when the cell is exposed to stress conditions to be a series of polypeptides acting as a molecular accompaniment to preserve cellular function against many pathogens. Giardia is a widespread parasite related to human diarrhea and other types of mammals. The current study was conducted in Dhi Qar Governorate for the period from the beginning of August 2018 to the end of June 2019. The study included the collection of 550 samples of people with diarrhea were examined using direct-examination method, iodine dyeing method, and salt-floating method to reveal samples of the Giardia parasite.
The aim of this study: determine Giardia isolates using the 18srRNA gene, determine the genotypes of G. lamblia in Dhi-Qar using Nested PCR technology based on amplificationTPI gene and then use PCR to detect the HSP70 gene in Giardia isolates.
Materials and Methods: In this diagnostic case control study that performed in Thi-Qar governorate \ Iraq between 2018-2020, Serological tests were performed on 96 samples (48 patients and 48 control). This study used the enzyme-linked immunoassay (ELISA) to measure the amount of HSP70 in the blood. the presence of the HSP70 gene in feces in the diagnosis using the 18SrRNA gene for molecular diagnosis, using PCR for Giardia lamblia detection. The study also included knowledge of genotypes using the TPI gene and found to carry genotypes A. Then the genes were sent for comparison with the global strains in the gene bank, which confirmed the presence of new strains based the18SrRNA gene sequences. The match rate ranged between the studied models and the recorded species (100-99%) and the species was registered in the NCBI with the following accession numbers MN124152.1 and MN124153 .1, MN124154.1, MN124155.1, MN124155. 1, MN124157.1, MN 124158.1, MN124159.1, MN124160.1, MN1241561.1.
Results: The results of the current study showed that the number of samples infected with intestinal parasites is 256 ( 46.55%) pateints , while 294 (53.45%)pateints are with dieriathat dye to other caused.The study showed the presence of five types of intestinal parasites prevalent in Dhi Qar Governorate, Giardia lamblia by 18.75%, antimopia histolytica by 58.59%, entomopia coli by 5.46%, heminoles nana by 3.9% and Enterobius vermicularis and there is a good relation between presence of Giardia lamblia The amount of HSP70 in the blood shows an increase in the amount of protein in infected people (20.53% ng / ml) while in samples Control (4.069% ng / ml) and the stool.
Conclusion: The current study shows that there is an increase in the rate of thermal shock protein in people with the parasite of Giardia lamblia as it showed the presence of a protein gene in the stools of the infected, also showed that the genetic model responsible for the infection in Dhi Qar province is model A.
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| Keyword:
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HSP70, Giardia Lamblia , Thi-Qar
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.210
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| Download:
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