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Oxalate oxidase: An Antilithiatic Enzyme from Staphylococcus pasteuri and its Degradation Studies
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| Author:
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NANDAKUMAR ARUMUGAM, SHIVA KUMAR VEERAKUMAR, SHRUTHI SARAVANAN, YAMINI VASUDEVAN, PRABHARKAR JERIK SATHYA RAJ
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| Abstract:
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Calcium oxalate (nephrolithiasis) is the most frequent type of kidney stone disease (KSD) affecting the urinary tract. In this study, the degradation and excretion of oxalate from body was carried out by utilizing oxalate oxidase producing bacterial strains. Bacterial strains were isolated from medical waste dumped soil and analyzed for its oxalate oxidase activity by using calcium oxalate, L-ascorbic acid, and oxalic acid and their ability to degrade the substrates was studied. Also, the optimized condition for the effective enzyme activity was found to be higher at pH - 5, temperature (40°C), substrates (Calcium oxalate & Oxalic acid), ZnO metal (0.01 mg/mL) and chelating agent (EDTA) of 0.01 mg/mL concentration. Further, ammonium sulphate precipitation of 50% saturation was performed and then partially purified by dialysis. The optimization for the partially purified enzyme activity was executed and the optimized conditions are temperature (400C), substrates (Oxalic acid), and pH (5). In vitro analysis of the kidney stone formation was tested for the partially purified enzyme and identified to be the potential source for the treatment of kidney stones.
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| Keyword:
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Nephrolithiasis, Calcium oxalate, Oxalate oxidase, Oxalic acid and Kidney stone.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.207
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| Download:
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