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Effect of meloxicam and its metal [Zn(II), Cu(II), Co(II), Ni(II)] complexes on viability and/or proliferation of human non-small cell lung cancer cells
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| Author:
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LORA DYAKOVA, DANIELA-CRISTINA CULITA, TANYA ZHIVKOVA, MILENA GEORGIEVA, ROSSEN SPASOV, GEORGE MILOSHEV, RENI KALFIN, GABRIELA MARINESCU, LUMINITA PATRON, RADOSTINA ALEXANDROVA
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| Abstract:
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The purpose of the present investigations was to study the effect of selective non-steroidal anti-inflammatory agent meloxicam and its metal [Zn(II), Cu(II), Co(II), Ni(II)] complexes on viability and proliferation of cultured A549 human non-small cell lung cancer (NSCLC) cells.
The investigations performed by thiazolyl blue tetrazolium bromide (MTT) test (the gold standard for cytotoxicity assay) revealed that applied at a concentration range of 5 – 500 µg/ml for 24h, 48h and 72h, the compounds examined express cytotoxic effect that is time- and concentration- dependent. Tested independently, meloxicam exhibits the lowest cytotoxicity. The effective concentrations of meloxicam and its metal complexes that reduce the amount of viable cells by 50% (CC50) as compared to the control (DMSO-control) were found to be lower in NSCLC A549 cells as compared to human non-tumor lung embryonic Lep-3 cells. Cu(II) complex of meloxicam was found to exhibit the highest cytotoxic activity that was further confirmed by double staining with acridine orange and propidium iodide, AnnexinV/FITC test and 3D colony-forming method.
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| Keyword:
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meloxicam, metal complexes, non-small cell lung cancer, cell cultures, cytotoxicity
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.216
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