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Production and characterization of polyhydroxybutyrate (PHB) from marine invertebrates
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| Author:
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N. EZHILRAJ, S. CHIPPY RAJ , S. MARY JOSEPHINE PUNITHA
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| Abstract:
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Biopolymers are formed by living organisms which are naturally occurring substance with high molecular weight. The study focussed on isolation of marine bacteria from Nereis sp. and screening for PHB producing isolates using Sudan Black B staining method. One out of seven isolates was chosen for extraction of PHB, spectral and chromatographic characterisation and was identified using 16S rRNA gene sequencing and phylogenetic analysis. Physiological characterisation, antibiotic resistance and sensitivity pattern was also studied. The FTIR spectrum showed presence of functional groups of carbine-methylene and also indicated presence of lipids. The potent polymer producing bacterium was identified as Bacillus paranthracis with 99 % homology and neighbour joining tree was also obtained.This study is a preliminary analysis that proposes the characterisation of PHB for the preparation of new eco-friendly bioplastic and also the bacterium Bacillus paranthracis could be used as a potent source for the manufacturing of biopolymer in large scale with further studies.
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| Keyword:
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Biopolymers; Polyhydroxybutyrate; FTIR; TLC; Phylogenetic analysis.
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| EOI:
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| DOI:
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