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Immunohistochemical Expression of (Zo-1 & GFAP) Antibody in the Sulcus Medianus Organum of Rat
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| Author:
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HAYDER AL, HAYDER J. KADHIM, HAIDER F. GHAZI
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| Abstract:
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The circumventricular organs (CVOs) are peculiar structures that border the brain ventricles, they are highly vascularized and lined by a specialized ependymal cells called tanycytes. There are seven CVOs that are generally characterized by deficient blood brain barrier and can be classified into two main groups (sensory& secretory CVOs).
The sulcus medianus organum (SMO) is a previously described CVO, is located at the floor of the fourth ventricle at the rostral part of median sulcus, little information is available about this region of the brain.
This research performed an immunohistochemical study considering the astrocytes and the ependymal lining of this region to explore the previous observations that suggest it as a CVO.
Twenty adult male rats (Rattus Norvegius Albinus) aged 3-6 months were used to study the immunohistochemistry of the SMO & the median eminence (ME) which was used as a control region since it was a known CVO lined by specialized ependymal cells (tanycytes).
The immunohistochemical study involved the use of (ZO-1 antibody) to detect the tight junctions and (GFAP antibody) to evaluate the distribution of the astrocytes and their foot processes in this region.
The immunohistochemical labeling findings showed a ZO-1 antibody reactivity that forms a continuous green line at the apical aspects of the ependymal lining of both SMO & ME.
The GFAP antibody labeling in the ME region was most marked at the lateral aspects at a sub-ependymal level while that of the SMO showed a sporadic distribution of the astrocytes.
Conclusively, the SMO region contain an ependymal lining cells that could be regarded as a tanycytes-like cells & The GFAP immunohistochemical reactivity in the region of SMO showed diffuse stellate astrocytes that may indicate the formation of glia limitans related to blood brain barrier in this region.
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| Keyword:
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Circumventricular Organs (CVO), Sulcus Medianus Organum (SMO), Median Eminence (ME), ZO-1 Antibody (Zonula Occludens-1 Antibody), GFAP Antibody (Glial Fibrilary Acidic Protein Antibody), Tanycytes.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.120
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