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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Molecular Study of Fluoroquinolones Resistance Staphylococcus Aureus Isolated from Different Clinical Sources

Author: SAFAA ATTA, EHAB D. SALMAN
Abstract: Fluoroquinolones are the most effective antibiotics against Staphylococcus aureus isolates. In hospitals, excessive use of antibiotics has led to the emergence of highly resistant strains of S. aureus isolates. The aim of this study was to the detection of mutations that-occur in the grlA gene encoding for DNA gyrase and responsible for fluoroquinolones resistance. Fifty clinical isolates were diagnosed as S. aureus according to bacteriological ways. The susceptibility test was performed to all bacterial isolates by disc diffusion method (DD) through using six fluoroquinolones antibiotics. From fifty isolates, twelve isolates (resistant) for all seven antibiotics. From the fifty isolates 12 resistant, 3 intermediate and 38 sensitive to three or more tested antibiotics, in addition to confirmed the resistant of S. aureus isolates by Minimum inhibitory concentration test. The main sources of S. aureus isolates were burn (10%), nose (16) wound (8%), ear (20%), operation room (10%), skin (6%), urine (8%) and throat (22%). A twelve resistant isolates were used to exam the mutations in grlA genes. In grlA gene, the direct sequence was identified seven mutations, 1 (14.2% silent mutation), 3 (42.9% missense mutations) and 3 (42.9 % insertion mutations) at various positions. Finally, all this-mutations in grlA gene are responsible for fluoroquinolones resistance as major mechanism.
Keyword: Staphylococcus Aureus, Fluoroquinolone, grlA.
DOI: https://doi.org/10.31838/ijpr/2020.12.03.118
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