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High Performance Thin Layer Chromatographic Estimation of Cefuroxime Axitel in Bulk and Pharmaceutical Formulation
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| Author:
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UPLOADED BY-ADMIN, MEENAXI M. MASTE, YUVARAJ D. MANDAVKAR, BHAT A.R
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| Abstract:
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A simple, fast, precise and accurate high performance thin layer chromatographic method has been developed for the
estimation of Cefuroxime axitel in dosage forms. The chromatographic separation was developed using a pre-coated silica
gel 60 GF254. TLC plate as stationary phase and a chromatogram was developed using chloroform: methanol: toluene (7:4:3
v/v/v) as mobile phase. Densitometric evaluation was performed at 289 nm. Cefuroxime axitel was resolved satisfactorily at
Rf values 0.72 ± 0.001. The developed method was validated for accuracy, linearity, precision, limit of detection and limit of
quantification and robustness as per the ICH guidelines. The calibration curve of HPTLC data was found to be linear
between 300 to 800 ng/spot for Cefuroxime axetil. The limit of detection and the limit of quantification for the Cefuroxime
axetil were found to be 9.85 ng/spot and 100.95 ng/spot. The proposed method can be successfully used to determine the
drug content of marketed formulation.
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| Keyword:
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Cefuroxime Axetil (CA), Tablet form, HPTLC.
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| EOI:
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| DOI:
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