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Hepatocytic Proteins in the Correction of Immune and Metabolic Disturbances in Experimental Chronic Alcohol Intoxication
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| Author:
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EKATERINA LITVINOVA, ANASTASIA V. KHARCHENKO, OLGA N. BUSHMINA, NIKOLAY A. KONOPLYA
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| Abstract:
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Aim: To determine corrective effects of proteins isolated from the culture fluids of allogeneic hepatocytes on immune and metabolic disturbances induced by chronic alcohol intoxication (CAI). Materials and Methods: The work was carried out with the participation of 120 Wistar rats. CAI was caused by daily intragastric introduction of 20% C2H5OH, at a dose 3 ml/kg (2.92 g/kg) for two months. Proteins of allogeneic hepatocytes (PAH) were isolated from the culture fluids of allogeneic hepatocytes (CFAH), obtained from newborn rats according to M. N. Berry and D. S. Friend method. CFAH and PAH were abdominally introduced (with a 24-hour interval) five times, calculated as 5 mg/kg of protein to allogeneic adult specimens with CAI from the 55th day of ethanol intoxication. Results: In the presence of CAI the development of cytolytic, cholestatic, cytotoxic, inflammatory syndromes, insufficiency of synthetic reactions of hepatocytes, activation of processes of lipid peroxidation, the disturbance of endoglobular metabolism, the development of oxidative stress, the suppression of adaptive and innate immunity has been revealed. The introduction of CFAH, undivided proteins of CFAH or proteins of AH with a molecular mass (MM) less than 130 kDa to the animals with CAI, normalizes or corrects the studied biochemical and immunological parameters. The proteins of CFAH with MM more than 130 kDa do not possess such effects. Conclusion: Taking into account high efficacy of PAH in the correction of immuno-metabolic disorders in CAI in the experiment, a promising direction in this research is the further purification of the effector protein substance to create hepatoprotective medicinal preparation on this basis.
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| Keyword:
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chronic alcoholic intoxication, immune-metabolic disorders, proteins of allogeneic hepatocytes.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.085
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