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Molecular study on Enterococcus faecalis isolated from Primary Endodontic infection
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| Author:
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HAJIR HUSSEIN, AHMED GHANIM ALHELAL, FATIMA MALIK ABOOD
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| Abstract:
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Back ground: The term “endodontic infections” refer to any infection occur in the dental root canal caused by bacteria mostly anaerobes .
Objective: To detect E.faecalis by using PCR assay based on 16SrRNA and culture technique, also to investigate the virulence factors of bacteria and to evaluate the influences of some antimicrobial agents on E.faecalis.
Materials and methods: Eighty Samples were collected for the current study from patients with mean age (33y), these samples was utilized to detect the existence of E.faecalis through multiple method specially traditional culture and PCR technique based on 16SrRNA gene and in vitro antimicrobial agents were used for screening the antimicrobial effect by Disk diffusion test (the Kirby-Bauer susceptibility test).
Results: The results showed about (75%) of the samples have positive results confirmed by PCR at molecular level, while with conventional bacteriological method, (68.8%) of the samples showed positive results. In vitro antimicrobial screening against E.faecalis showed that all isolates were sensitive to vancomycin, amoxicillin, azithromycin and ciprofloxacin. In contrast, most isolates revealed a moderate sensitivity to co-amoxiclav. And most isolates seems to be resistant to the other antibiotics including: cloxacillin, ceftriaxone, metronidazole, cefixime, lincomycin. In addition, the current study found about (60%) of isolates was able to produce extracellular protease, about (90%) isolated have ability to produce gelatinase and about 100% of isolates were able to produce biofilm and almost all bacteria have the capability of adhere to oral epithelial cells.
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| Keyword:
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Enterococcus faecalis, Endodontic infection, PCR.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.059
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| Download:
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